The aim of this study is to determine the level of regeneration of five types of cells and stem cells in patients after exposure to Fluoroquinolone antibiotics. These cell culture results will allow us to continue work on possible therapy for patients with Fluoroquinolone Associated Disability (FQAD) as well as learn how to possibly prevent ADR’s to these drugs.
We are currently studying the impact of Cipro, Levaquin, and Moxicillin, in comparison to chemotherapy drug, Afatinib, on the following type of cells:
Following peer review, the results of this study will be co-published and posted here.
Fluoroquinolones are a group of antibiotics that are widely prescribed to treat a variety of bacterial infections. While the intended target is bacteria, the Fluoroquinolones are also known to interact with the human genomic system by conjugating with Deoxyribonucleic acid (DNA). Given the crucial role of DNA in human body function, any damage may have serious health consequences.
Our study was conducted with subjects suffering from Fluoroquinolone Associated Disability (“FQAD”):
Determine if conjugates form between Levofloxacin, Ciprofloxacin and DNA.
(A1). Study for Fluoroquinolone DNA adduct. Human blood samples [50+ participants/samples from suspected cases FQAD]
(A2). Study for Fluoroquinolone mtDNA adduct. Human blood samples [10 participants/samples from suspected cases FQAD]
(A3). Study for Fluoroquinolone DNA adduct. Human blood samples [50 participants from suspected cases FQAD, plus samples/4 children who had not taken a Fluoroquinolone, but their mothers did before or during pregnancy]
(A4). Control group who had never taken a Fluoroquinolone
-description of Fluoroquinolone DNA adduct
- methodology and strategy of study
- standard spectrogram Fluoroquinolone only
- spectrogram of control blood sample
- spectrogram of control blood sample after addition of Fluoroquinolone
- spectrograms of Fluoroquinolone adduct to genomic and mitochondrial DNA
- diagrams showing the mechanism of Fluoroquinolone adduct to DNA
- summary, test results and substantiation
In the link below, you will find a sample of the results on the Levofloxacin adduct to mitochondria and genomic DNA along with methods used during this study:
On the above test, results showed that an adduct molecule could be formed between Levofloxacin and control blood. The adduct was structurally characterized, using Mass Spectrometry (HPLC-MS/MS). Although the exact configuration of the Adduct was not obvious in the Patient's DNA, the ions lend support to its origin to be Levofloxacin and Guanine (a portion of DNA molecule).
Out of the numerous test results we had patient permission to access, all showed genomic (nuclear) and mitochondrial DNA Fluoroquinolone adducts.
DNA damage was also detectable decades after the original exposure by both single or multiple oral doses, IV or ear drop forms.
Our in vitro test manifested DNA damage in just 3.61 minutes after antibiotic introduction and a second adduct appears at the 13.12 minute mark; the same damage is seen with Cipro.
We are aware of only two other tests performed on Fluoroquinolone DNA adducts in humans, which were completed using small in vivo genomic samples, in various laboratories done in 2014 and 2019.
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